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ac tub  (Proteintech)


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    Proteintech ac tub
    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin <t>O</t> <t>staining</t> of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) <t>and</t> <t>AC-Tub</t> (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.
    Ac Tub, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tubulin/acetylated+Tubulin(Lys40)+Antibody/pmc12927103-197-6-7
    Average 95 stars, based on 176 article reviews
    ac tub - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury"

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.01.026

    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.
    Figure Legend Snippet: Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

    Techniques Used: Staining, Marker

    Related Articles

    Incubation:

    Article Title: GSK3β as a potential regulator in AML: A pan-cancer multi-omics analysis
    Article Snippet: .. Membranes were incubated with the following primary antibodies: anti-GSK-3β(Proteintech, 22104–1-AP, Wuhan, China)a, anti-c-Myc(Proteintech, 10828–1-AP, Wuhan, China), PAPR1(Proteintech, 66520–1-Ig, Wuhan, China)and anti-Tubulin (Proteintech, 80762–1-RR, Wuhan, China); anti-p-GSK3β(Cell Signaling Technology, 5558T, Danvers, MA, USA), anti-CCND1 (Cell Signaling Technology, 55506S, Danvers, MA, USA). .. Following three TBST washes, protein bands were detected using HRP-conjugated secondary antibodies (Beyotime, A0216 and A0208, China) through 1-hour room temperature incubation.

    Article Title: RUVBL2 Regulates Microglia Metabolic Reprogramming to Mediate Stress Granules Aggregation Exacerbating Postoperative Delirium in Aged Mild Cognitive Impairment Rats.
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies: anti- RUVBL2 (1:1000, Catalog Number: ab19027, Abcam, Cambridge, UK), antiα- Ketoglutarate Dehydrogenase (OGDH) (1:1000, Catalog Number: 15212- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Pyruvate Kinase 2 (PKM2) (1:1000, Catalog Number: 15822- 1- AP, Proteintech Group Inc., Wuhan, China), anti- CD86 (1:1000, Catalog Number: 13395- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Arginase 1 (Arg1) (1:1000, Catalog Number: 16001- 1- AP, Proteintech Group Inc., Wuhan, China), and anti- Tubulin (1:50,000, Catalog Number: 10094- 1- AP, Proteintech Group Inc., Wuhan, China) as the internal reference. .. After three washes with Tris- buffered saline containing Tween- 20 (TBST), the membranes were incubated with the appropriate secondary antibodies at room temperature for 1 h. Following additional washing, the PVDF membranes were exposed to enhanced chemiluminescence (ECL) reagent (Elabscience Biotechnology Co. Ltd., Wuhan, China) for visualization and imaging.

    Article Title: Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activity.
    Article Snippet: Western blot Cells were treated as those in quantitative RT-PCR and protein lysates were separated by 10% SDS-PAGE gels and transferred onto a nitrocellulose filter membrane (NC) membrane (Pall,66485), blocked with 5% non-fat milk in phosphate buffered solution with 0.1% Tween 20 (PBST). .. Primary antibodies were incubated at 4 °C overnight, including Anti-STIM1 (Cell Signaling Technology, 5668, 1:3000), Anti-ORAI1 (Santa Cruz, sc-377281, 1:500), Anti-TRPC1 (Proteintech, 19482-1-AP, 1:2000), Anti-E-Cadherin (Proteintech, 60335-1-Ig, 1:2000), Anti-ZO1 (Cell Signaling Technology, 13663, 1:3000), Anti-EPCAM (Proteintech, 21050-1-AP, 1:2000), Anti-Vimentin (Cell Signaling Technology, 5741S, 3:1000), Anti-N-Cadherin (Proteintech, 66219- 1-Ig, 1:2000), Anti--SMA (Abcam, ab7817, 1:3000), Anti--Tubulin (Proteintech, 11224-1-AP, 1:2000), Anti-acetyl--Tubulin (Proteintech, 66200-1-Ig, 1:2000), and Anti-GAPDH (Proteintech, 60004-1-1g, 1:10000). ..

    Article Title: GSK3β as a potential regulator in AML: A pan-cancer multi-omics analysis.
    Article Snippet: .. Membranes were incubated with the following primary antibodies: anti-GSK-3β(Proteintech, 22104–1-AP, Wuhan, China)a, anti-c-Myc(Proteintech, 10828–1- AP, Wuhan, China), PAPR1(Proteintech, 66520–1-Ig, Wuhan, China)and anti-Tubulin (Proteintech, 80762–1-RR, Wuhan, China); anti-p-GSK3β(Cell Signaling Technology, 5558T, Danvers, MA, USA), anti-CCND1 (Cell Signaling Technology, 55506S, Danvers, MA, USA). .. Following three TBST washes, protein bands were detected using HRP-conjugated secondary antibodies (Beyotime, A0216 and A0208, China) through 1-hour room temperature incubation.

    Western Blot:

    Article Title: An interferon-independent innate immune response to double stranded RNA in embryonic stem cells
    Article Snippet: After another series of TBST washes, protein bands were visualized using an enhanced chemiluminescence (ECL) detection kit (34577, ThermoFisher Scientific) and imaged with a ChemiDocTM MP Imaging System (Bio-Rad). .. The following antibodies were used for WB or IP analysis: anti-Tubulin (1:5000 for WB, 66031-1-Ig, Proteintech), anti-Actin (1:5000 for WB, 66009-1-Ig, Proteintech), anti-p53 (1:5000 for WB and 2 μg for each IP, 10442-1-AP, Proteintech), anti-p53 (1:1000 for WB, ab77813, Abcam), anti-Puromycin (1:1000 for WB, MAB3343, Sigma-Aldrich), anti-Stat1 (1:1000 for WB and 2 μg for each IP, 14994S, Cell Signaling Technology), anti-p701-Stat1 (1:1000 for WB, 9167S, Cell Signaling Technology), anti-Dhx9 (1:5000 for WB and 2 μg for each IP, 17721-1-AP, Proteintech), anti-Mdm2 (1:1000 for WB, 66511-1-Ig, Proteintech), anti-Ubiquitin (1:1000 for WB, sc-8017, Santa Cruz Biotechnology), anti-Cul4A (1:1000 for WB, 14851-1-AP, Proteintech), anti-Ddb1 (1:1000 for WB, PA5-21282, Invitrogen), anti-Ddb2 (1:1000 for WB, ab77765, Abcam), anti-Rbx1 (1:1000 for WB, 14895-1-AP, Proteintech), anti-Gapdh (1:5000 for WB, 60004-1-Ig, Proteintech), anti-J2 (2 μg for each IP, 76651L, Cell Signaling Technology), anti-Tcf4 (1:1000 for WB, 05-511, Millipore), mouse control IgG (2 μg for each IP, B900620, Proteintech), and rabbit control IgG (2 μg for each IP, B900610, Proteintech); HRP conjugated anti-mouse (1:5000 for WB, 31430, Invitrogen) or rabbit (1:5000 for WB, 31460, Invitrogen) IgG (H+L) was used as the secondary antibody for WB analysis. ..

    Control:

    Article Title: An interferon-independent innate immune response to double stranded RNA in embryonic stem cells
    Article Snippet: After another series of TBST washes, protein bands were visualized using an enhanced chemiluminescence (ECL) detection kit (34577, ThermoFisher Scientific) and imaged with a ChemiDocTM MP Imaging System (Bio-Rad). .. The following antibodies were used for WB or IP analysis: anti-Tubulin (1:5000 for WB, 66031-1-Ig, Proteintech), anti-Actin (1:5000 for WB, 66009-1-Ig, Proteintech), anti-p53 (1:5000 for WB and 2 μg for each IP, 10442-1-AP, Proteintech), anti-p53 (1:1000 for WB, ab77813, Abcam), anti-Puromycin (1:1000 for WB, MAB3343, Sigma-Aldrich), anti-Stat1 (1:1000 for WB and 2 μg for each IP, 14994S, Cell Signaling Technology), anti-p701-Stat1 (1:1000 for WB, 9167S, Cell Signaling Technology), anti-Dhx9 (1:5000 for WB and 2 μg for each IP, 17721-1-AP, Proteintech), anti-Mdm2 (1:1000 for WB, 66511-1-Ig, Proteintech), anti-Ubiquitin (1:1000 for WB, sc-8017, Santa Cruz Biotechnology), anti-Cul4A (1:1000 for WB, 14851-1-AP, Proteintech), anti-Ddb1 (1:1000 for WB, PA5-21282, Invitrogen), anti-Ddb2 (1:1000 for WB, ab77765, Abcam), anti-Rbx1 (1:1000 for WB, 14895-1-AP, Proteintech), anti-Gapdh (1:5000 for WB, 60004-1-Ig, Proteintech), anti-J2 (2 μg for each IP, 76651L, Cell Signaling Technology), anti-Tcf4 (1:1000 for WB, 05-511, Millipore), mouse control IgG (2 μg for each IP, B900620, Proteintech), and rabbit control IgG (2 μg for each IP, B900610, Proteintech); HRP conjugated anti-mouse (1:5000 for WB, 31430, Invitrogen) or rabbit (1:5000 for WB, 31460, Invitrogen) IgG (H+L) was used as the secondary antibody for WB analysis. ..

    other:

    Article Title: BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia.
    Article Snippet: Protein band intensities were measured from individual lanes using ImageJ software, subtracted by background signals, and then normalized to controls.



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    Image Search Results


    Melatonin dose modulates NSCs lineage commitment, viability, oxidative stress, and mitochondrial membrane potential at day 5. (A) Representative immunofluorescence images of TUJ1 with Nestin in NSCs cultured within the cell-laden MT/BEM matrix and exposed to melatonin (0, 25, 50, 75, 100 μM) for 5 days (scale bar, 50 μm). (B) Representative images of GFAP with Nestin under the same conditions (scale bar, 50 μm). (C) Representative images of Olig2 with Nestin (scale bar, 50 μm). (D) Percentages of TUJ1(+), GFAP (+), and Olig2(+) cells relative to total nuclei (DAPI) (n = 10 fields/group). (E) RT-qPCR of TUJ1, GFAP, and Olig2 normalized to GAPDH and expressed as fold change versus control (ΔΔCt) (n = 6). (F) Live/Dead staining (Calcein AM/EthD-1) at day 5. (G) Western blots of TUJ1 and GFAP with GAPDH loading control. (H) Densitometry of TUJ1/GAPDH and GFAP/GAPDH (n = 3 independent experiments). (I) ROS staining by DCFH-DA with Rosup as the positive control. (J) Quantification of ROS fluorescence intensity (n = 10 fields/group, compared to control). (K) JC-1 staining of mitochondrial membrane potential (ΔΨm) with CCCP as the positive control for depolarization. (L) JC-1 red/green ratio (n = 10 fields/group, compared to control). Statistical analysis: Data are presented as mean ± SD. one-way ANOVA with Holm–Sidak's multiple comparisons for multi-group datasets (D, E, J, L); unpaired two-tailed t -test for the two-group comparison (H). Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Melatonin dose modulates NSCs lineage commitment, viability, oxidative stress, and mitochondrial membrane potential at day 5. (A) Representative immunofluorescence images of TUJ1 with Nestin in NSCs cultured within the cell-laden MT/BEM matrix and exposed to melatonin (0, 25, 50, 75, 100 μM) for 5 days (scale bar, 50 μm). (B) Representative images of GFAP with Nestin under the same conditions (scale bar, 50 μm). (C) Representative images of Olig2 with Nestin (scale bar, 50 μm). (D) Percentages of TUJ1(+), GFAP (+), and Olig2(+) cells relative to total nuclei (DAPI) (n = 10 fields/group). (E) RT-qPCR of TUJ1, GFAP, and Olig2 normalized to GAPDH and expressed as fold change versus control (ΔΔCt) (n = 6). (F) Live/Dead staining (Calcein AM/EthD-1) at day 5. (G) Western blots of TUJ1 and GFAP with GAPDH loading control. (H) Densitometry of TUJ1/GAPDH and GFAP/GAPDH (n = 3 independent experiments). (I) ROS staining by DCFH-DA with Rosup as the positive control. (J) Quantification of ROS fluorescence intensity (n = 10 fields/group, compared to control). (K) JC-1 staining of mitochondrial membrane potential (ΔΨm) with CCCP as the positive control for depolarization. (L) JC-1 red/green ratio (n = 10 fields/group, compared to control). Statistical analysis: Data are presented as mean ± SD. one-way ANOVA with Holm–Sidak's multiple comparisons for multi-group datasets (D, E, J, L); unpaired two-tailed t -test for the two-group comparison (H). Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Membrane, Immunofluorescence, Cell Culture, Quantitative RT-PCR, Control, Staining, Western Blot, Positive Control, Fluorescence, Two Tailed Test, Comparison

    Three-dimensional immunofluorescence and transcriptomic profiling of melatonin-treated NSCs. (A) Representative 3D confocal reconstructions of NSCs networks cultured for 5 days in BEM or MT/BEM hydrogels, immunostained for Nestin, TUJ1, GFAP and OLIG2 with DAPI nuclear counterstain. Scale bar: 50 μm. (B-C) Quantification of neurite outgrowth showing total neurite length (μm) (B) and neurite filament area (μm 2 ) (C) per field of view. (D) Quantification of astroglial differentiation expressed as GFAP + area (% of ROI). (E) Quantification of oligodendroglial lineage commitment expressed as OLIG2 + cells (% of DAPI + nuclei). Data are presented as mean ± SD. Statistical significance was assessed using an unpaired two-tailed t -test; ∗p < 0.05, ∗∗p < 0.01 versus NSCs@BEM.

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Three-dimensional immunofluorescence and transcriptomic profiling of melatonin-treated NSCs. (A) Representative 3D confocal reconstructions of NSCs networks cultured for 5 days in BEM or MT/BEM hydrogels, immunostained for Nestin, TUJ1, GFAP and OLIG2 with DAPI nuclear counterstain. Scale bar: 50 μm. (B-C) Quantification of neurite outgrowth showing total neurite length (μm) (B) and neurite filament area (μm 2 ) (C) per field of view. (D) Quantification of astroglial differentiation expressed as GFAP + area (% of ROI). (E) Quantification of oligodendroglial lineage commitment expressed as OLIG2 + cells (% of DAPI + nuclei). Data are presented as mean ± SD. Statistical significance was assessed using an unpaired two-tailed t -test; ∗p < 0.05, ∗∗p < 0.01 versus NSCs@BEM.

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Immunofluorescence, Cell Culture, Two Tailed Test

    Molecular validation of neural repair and mechanism activation in spinal cord tissue. Western blot and qPCR analyses of spinal cord tissue lysates from Sham, SCI, BEM, NSCs@BEM, and NSCs@MT/BEM groups. (A) Representative Western blots for the neuronal marker TUJ1 and the glial scar marker GFAP. (B) Representative Western blots for phosphorylated AMPK (p-AMPK), phosphorylated ACC (p-ACC), and their respective total proteins. (C) Representative Western blots for the five oxidative phosphorylation (OXPHOS) complex subunits. (D) Densitometric quantification of TUJ1 and GFAP protein levels. (E) Densitometric quantification of the p-AMPK/total AMPK and p-ACC/total ACC ratios. (F) Densitometric quantification of OXPHOS complex protein levels. (G) Relative mRNA expression of neural markers (TUJ1, GFAP, Olig2) and key mitochondrial biogenesis regulators (Ppargc1a, Tfam) determined by qPCR. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA with Holm–Sidak's multiple comparisons test. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Molecular validation of neural repair and mechanism activation in spinal cord tissue. Western blot and qPCR analyses of spinal cord tissue lysates from Sham, SCI, BEM, NSCs@BEM, and NSCs@MT/BEM groups. (A) Representative Western blots for the neuronal marker TUJ1 and the glial scar marker GFAP. (B) Representative Western blots for phosphorylated AMPK (p-AMPK), phosphorylated ACC (p-ACC), and their respective total proteins. (C) Representative Western blots for the five oxidative phosphorylation (OXPHOS) complex subunits. (D) Densitometric quantification of TUJ1 and GFAP protein levels. (E) Densitometric quantification of the p-AMPK/total AMPK and p-ACC/total ACC ratios. (F) Densitometric quantification of OXPHOS complex protein levels. (G) Relative mRNA expression of neural markers (TUJ1, GFAP, Olig2) and key mitochondrial biogenesis regulators (Ppargc1a, Tfam) determined by qPCR. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA with Holm–Sidak's multiple comparisons test. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Biomarker Discovery, Activation Assay, Western Blot, Marker, Phospho-proteomics, Expressing

    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

    Journal: Bioactive Materials

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

    doi: 10.1016/j.bioactmat.2026.01.026

    Figure Lengend Snippet: Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

    Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

    Techniques: Staining, Marker